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Literature summary extracted from

  • Stengel, C.; Newman, S.P.; Day, J.M.; Tutill, H.J.; Reed, M.J.; Purohit, A.
    Effects of mutations and glycosylations on STS activity: a site-directed mutagenesis study (2008), Mol. Cell. Endocrinol., 283, 76-82.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.6.2 His6-tagged wild-type STS cDNA subcloned into pCEP4, transfected into 293-EBNA cells, cell line with stable overexpression of STS-His6 selected for production of tagged wildtype STS, subsequent specific antibody production, mutated STS is transfected into human MCF-7 cells and monkey COS-1 cells to test for STS activity by immunoblotting Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.6.2 DELTA1-21 lacks 21 N-terminal amino acids, that encode the signal peptide, reduced STS activity Homo sapiens
3.1.6.2 DELTA185-583 lacks 399 C-terminal amino acids, reduced STS activity Homo sapiens
3.1.6.2 H136F point mutation in histidine 136, reduced STS activitiy Homo sapiens
3.1.6.2 H136W point mutation in histidine 136, reduced STS activity Homo sapiens
3.1.6.2 H136Y point mutation in histidine 136, reduced STS activity Homo sapiens
3.1.6.2 additional information site directed mutagenisis is introduced using pALTERMax kit Homo sapiens
3.1.6.2 N259Q point mutation in asparagine 259 glycosylation site, reduced STS activity Homo sapiens
3.1.6.2 N333Q point mutation in other potential glycosylation site asparagine 333, no reduced STS activity Homo sapiens
3.1.6.2 N459Q point mutation in other potential glycosylation site asparagine 459, no reduced STS activity Homo sapiens
3.1.6.2 N47Q point mutation in asparagine 47 glycosylation site, reduced STS activity Homo sapiens
3.1.6.2 N47Q/N259Q double mutation in asparagine 47 and 259 glycosylation sites, reduced STS activity Homo sapiens
3.1.6.2 N54Q point mutation in in asparagine 54 non-glycosylation site , no reduced STS activity Homo sapiens
3.1.6.2 P212G point mutation in proline 212, no reduced STS activity Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.6.2 endoplasmic reticulum
-
 Homo sapiens 5783
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.6.2 61000
-
 SDS-PAGE, immunoblot analysis with specific antibody Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.6.2 estrone sulfate Homo sapiens
-
 estrone + sulfate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.6.2 Homo sapiens P08842
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.6.2 glycoprotein 4 potential glycosylation sites Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.6.2 affinity chromatography with NiCl2-primed HisTrap Chelating columns and concentrated 6-fold using ICON concentrators Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.6.2 HEK-293-EBNA cell human embryonic kidney carcinoma cell line selected for overexpression of transfected His6-tagged STS cDNA to aid purification of the His6-tagged STS protein and subsequent antibody production in mice and rabbits Homo sapiens
-
3.1.6.2 JEG-3 cell choriocarcinoma cell line used for expression test for transfection experiments Homo sapiens
-
3.1.6.2 MCF-7 cell ER positive human breast cancer cells, used for transfection and activity experiments, shows STS activity by itself in contrast to monkey COS-1 cells used for the same experiments Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.6.2 estrone sulfate
-
 Homo sapiens estrone + sulfate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.6.2 steroid sulfatase
-
 Homo sapiens
3.1.6.2 STS
-
 Homo sapiens